Strategy for Controlling Preferential Amplification and Avoiding False Negatives in PCR Typing
نویسندگان
چکیده
منابع مشابه
Strategy for controlling preferential amplification and avoiding false negatives in PCR typing.
The use of the PCR method for routine testing has increased dramatically during recent years. Most assays involve co-amplification either of an internal control, of several alleles at a given locus or of a variety of bands produced by low-stringency primer annealing. In such multiplex reactions, certain products will often amplify preferentially. Amplimers that are more sensitive can be outcomp...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1996
ISSN: 0736-6205,1940-9818
DOI: 10.2144/96216rr03